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Central Analytical Laboratory

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Overview

The Central Analytical Laboratory (CALab, B108) is well equipped with a number of sophisticated analytical instruments for the regular course works and research related activities. The following instrumental facilities as already established and available for services. 
 
 
 
State-of-the-Art Facilities and Specifications

  • 1) FIELD EMISSION SCANNING ELECTRON MICROSCOPY (FE-SEM)

           Field emission scanning electron microscopy (FE-SEM)

    FE-SEM is used to obtain topographical morphology and elemental information at magnifications of 10x to 300,000x, with virtually unlimited depth of field.  The instrument has a high resolution of 1.5 to 2 nm. The specifications allow one to image non-conducting samples as well, when imaged under inverse bias mode.

    (a)    Make: FEI

    (b)   Model: Apreo LoVac

    (c)    Retractable STEM 3+ Detector, DBS Detector

    (d)    Aztec Standard EDS system - resolution 127 eV on Mn-kα

    Leica Ultra Microtome EM UC7 (Sputter Coater)
     
     

  • 2) NUCLEAR MAGNETIC RESONANCE (NMR) – 400 MHZ

            Nuclear magnetic resonance (NMR) – 400 MHz

    NMR is a powerful technique to elucidate the structure of organic molecules and inorganic complexes. The instrument has the capability of measuring liquid (solution) as well as solid samples (using solid state probe). A variety of NMR active nuclei can be studies using the instrument.

    (a)    Make: Bruker

    (b)   Model: AV NEO (400 MHz)

    (c)    Multiprobe and low temperature in-built up to -35

    (d)    Magnet system: ASCEND™ 400 MHz/ 54 mm Long Hold Time Magnet operation field at 9.4 Tesla

    (e)    Auto-sampler

    (f)     NMR Tubes: 7” x 5.0 mm with coded caps 

     

  • 3) POWDER XRD - RIGAKU ULTIMA-IV

            POWDER XRD - Rigaku ULTIMA-IV

    X-ray diffraction (XRD) is an analytical technique primarily used for phase identification and crystallite size of a crystalline material. The instrument is capable of small angle X-ray scattering (SAXS) and variable temperature XRD measurements.

    (a)    Make: Rigaku

    (b)   Model: ULTIMA-IV

    (c)    Attachments: Powder, SAXS, High temperature (RT- 1000 ), Capillary, Thin film

    (d)    Measuring Circle Diameter: 1 cm x 1 cm x 0.5 mm, 1cm x 1cm x 0.3 mm  

    (e)    Angle range: 5-100°

    (f)     X-ray source: Copper

    Detector: Scintillation counter and dTex detector

  • 4) SINGLE CRYSTAL XRD - XTALAB

           SINGLE CRYSTAL XRD - XTALAB

    Single-crystal X-ray Diffraction measurement provides detailed information about the internal lattice of crystalline substances, including unit cell dimensions, bond-lengths, bond-angles, and details of site-ordering. With single-crystal refinement, one can interpret and refine the data to obtain the crystal structure.

    (a)    Make: Rigaku oxford

    (b)   Model: XTALAB

    (c)    Source: Mo (50mA, 40kV), Cu (50mA, 0.6kV)

    (d)    Detector: Dectris Pilatus 3R 200K-A

    (e)    Software: CrysAlisPro XtaLAB

    (f)     Cryo system: up to -180

    Dynamic range: >1,000,000:1 per pixel 
     

  • 5) LASER SCANNING CONFOCAL MICROSCOPE (LSCM) DMI

           LASER SCANNING CONFOCAL MICROSCOPE (LSCM) DMI8

    This technique allows one to capture multiple two-dimensional images at different depths in a sample and enables their construction into three-dimensional images. It is widely used in various disciplines such as cell biology, genetics, microbiology, developmental biology, pharmacy and nanotechnology.

    (a)    Make: Leica

    (b)   Model: TCS SP8

    (c)    Microscope: DMi8

    (d)    Magnification: 10x, 20x, 40x, 63x

    (e)    Lasers: 405, 458, 488, 514, 561, 594, 633 nm

    (f)     Detector: PMT 1, PMT 2, HyD 3, PMT 4 and PMT Trans

    Software: LAS X
     

  • 6) UNIVERSAL TESTING MACHINE (UTM)

    universal testing machine (UTM)

    The universal testing machine is a testing system designed to determine the mechanical properties of the specimens of various types of materials such as metals, polymers, elastomers, ceramics, biomaterials and composites, as per ASTM standards. This is achieved through mechanical loading by an electromechanical drive.

     

    Make: Zwick Roell Z100

    Load Cells: 100 kN and 5 kN

    Testing: Tension, Compression and Flexure

    Extensometer: Contact as well as Laser (Green)

    Operation: Constant engineering as well as true strain rate conditions

    Temperature chamber: -170 °C to 200 °C

    High-temperature furnace: up to 1200 °Celsius

     

  • 7) CIRCULAR DICHROISM (CD) SPECTROPHOTOMETER J-1500

         CIRCULAR DICHROISM (CD) SPECTROPHOTOMETER J-1500

    Circular dichroism (CD) measures the difference in absorbance of right- and left-circularly polarized light (rather than the commonly used absorbance of isotropic light) by a substance. The CD spectra are widely used to study the secondary structures of biological samples such as peptides and proteins.

    (a)    Make: Jasco

    (b)   Model: J-1500

    (c)    Software: Spectra Manager

    (d)    Detector: PMT

    (e)    Wavelength range: 190-800 nm (UV-Vis)

    Cuvettes: 1mm, 2mm, 5mm, 10mm
     

  • 8) ENERGY DISPERSIVE X-RAY FLUORESCENCE (ED-XRF) EPSILON-1

          Energy dispersive X-ray Fluorescence (ED-XRF) EPSILON-1

    ED-XRF is utilized in the identification and quantification of elements in a substance. The characteristic X-ray Fluorescence radiation intensity from the sample is compared with the standard library and calibration curves.

    (a)    Make: PANalytical 

    (b)   Model: Epsilon-1 (ED-XRF)

    (c)    Element range: Sodium to Americium

    Liquid and Solid 
     

  • 9) LIQUID CHROMATOGRAPHY- MASS SPECTROSCOPY(LC-MS-8040)

            LIQUID CHROMATOGRAPHY- MASS SPECTROSCOPY(LC-MS-8040)

    This technique is typically used to separate and analyze biological, organic, and inorganic compounds commonly found in a mixture. The technique also helps in identifying unknown compounds in complex sample matrices and quantify low level proteins, contaminants, pesticides, or drug metabolites with accuracy and reliability.

    (a)    Make: Shimadzu

    (b)   Model: LCMS 8040

    (c)    Modes: ESI and APCI

    Triple Quadrupole upto 2000 m/z value
     

  • 10) HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) - 2

          HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) - 2

    This chromatographic technique is an advanced version of liquid chromatography that is used to separate, identify, and quantify the components in a mixture of organic molecules.

    (a)    Make: Shimadzu

    (b)   Model: UFLC

    (c)    Fluorescence detector

    Second instrument: PDA detector
     

  • 11) GAS CHROMATOGRAPHY GC-2010 PLUS

            GAS CHROMATOGRAPHY GC-2010 PLUS

    Gas chromatography (GC) is useful in separating and analyzing compounds that vaporize without decomposition. Like liquid chromatography, GC is used to test the purity of a particular substance, or separate and quantify the different components of a mixture.

    (a)    Make: Shimadzu

    (b)   Model: GC-2010 PLUS

    (c)    Column: FID, TCD

    (d)    Max pressure: 970 kPa

    (e)    Max flow rate: 1200 ml/min

    (f)     Temperature range: RT to 400

    (g)    Auto Injector

    (h)   Carrier gas: Helium, Nitrogen, carbon dioxide 

     

  • 12) ATOMIC ABSORPTION SPECTROSCOPY AA-7000

         ATOMIC ABSORPTION SPECTROSCOPY AA-7000

    Atomic absorption spectroscopy is used for the identification and quantification of metals in ultra-low concentration present in different solutions.

    (a)    Make: Shimadzu

    (b)   Model: AA7000

    (c)    Lamps: 16 (Hg, Pd, As, Pb, Fe, Cd, Pt, Zn, Ni, Cr, Co, Al, Na, K, Cu, Ru, Ca)

    (d)    Lamp mode: EMISSION, NON-BGC, BGC-D2, BGC-SR

    (e)    Wavelength range: 185 nm to 900 nm

    (f)     Temperature range: RT to 3000

    (g)    Carrier gas: Ar, Acetylene, Nitrous Oxide

    (h)   Detection limit: ppm level

     

  • 13) DIFFERENTIAL SCANNING CALORIMETER DSC-60

          DIFFERENTIAL SCANNING CALORIMETER DSC-60

    This instrument measures the thermal fluctuations as a function of temperature in a sample. This is used to follow physical and chemical changes such as melting, crystallization, glass transition, endothermic and exothermic reactions.

    (a)    Make: Shimadzu

    (b)   Model: DSC-60

    (c)    Temperature range: RT to 500

    (d)    Measurement range: RT to 500

    (e)    Heating rate: 5,10,15,20 /min

    (f)     Cooling rate: 5,10,15,20 /min

    (g)    Atmosphere: Zero air, Nitrogen

    (h)   Flow rate: 50,100 ml/min

     

  • 14) DIFFERENTIAL THERMOGRAVIMETRIC ANALYSIS DTG-60

          DIFFERENTIAL THERMOGRAVIMETRIC ANALYSIS DTG-60

    Thermogravimetric analysis (TGA) provides information about thermal stability and degradation pattern of organic, inorganic and polymeric samples. Differential thermal analysis (DTA) simultaneously reveals the enthalpy of the physical and chemical changes as a function of temperature.

    (a)    Make/Model: Shimadzu DTG-60

    (b)   Temperature range: RT- 1000

    (c)    TG measurement range: upto 1000

    (d)    DTA measurement range: upto 1000

    (e)    Programmable rate: 5,10,15,20 /min

    (f)     Atmosphere: Nitrogen, Zero Air

    (g)    Flow rate: 50,100 ml/min

    Sensitivity of microbalance: 0.1 microgram
     

  • 15) Brunauer-Emmett-Teller (BET) Surface Area Analyzer

            Brunauer-Emmett-Teller (BET) Surface Area Analyzer:

    BET Surface Area Analyzer measures the specific surface area of powders, solids and granules. In addition, the technique also gives other valuable information such as pore size, pore size distribution, pore diameter and pore volume.

    (a)    Make: Microtrac Bel

    (b)   Model: BEL SORP mini II

    (c)    Gases: Helium, Nitrogen, carbon dioxide

    Software: BEL SORP mini
     

  • Other Instrumental Facilities

    1. UV-VIS SPECTROPHOTOMETER V-650 (190-900 nm)

    (a)    Make: Jasco

    (b)   Model: V-650

    (c)    Wavelength range: 190-900 nm

    (d)    Double beam system with single monochromator

    (e)    Wavelength accuracy: +/- 0.2 nm

    (f)     Repeatability: +/- 0.05

    (g)    Software: Spectra Manager

     

    2.       SPECTROFLUOROMETER FP-6300 (190-900 nm)

    (a)    Make: Jasco

    (b)   Model: FP-6300

    (c)    Wavelength range: 190-900 nm

     

    1. DIGITAL POLARIMETER P-2000

    (a)    Make: Jasco

    (b)   Model: P-2000

    (c)    Wavelength: 325, 365, 405, 436, 546, 589, 633 nm

    (d)    Lamp: Sodium Lamp, Halogen lamp

    (e)    Wavelength range: UV-Vis-NIR

    (f)     Software: Spectra Manager

     

    1. FOURIER TRANSFORM INFRARED SPECTROMETER FTIR-4200

    (a)    Make: Jasco

    (b)   Model: FTIR-4200

    (c)    Spectral range: 400-4000 nm

    (d)    Resolution: +/- 0.5

    (e)    Wavenumber accuracy: +/- 0.01 cm-1  

    (f)     Ge/KBr beam splitter

    (g)    DLATGS Detector

    (h)   DRA-81

    (i)      Solid / liquid samples about 2 mg. Solvent should be specified for measurement in solution phases

    1. UV-VIS-NIR SPECTROPHOTOMETER UV- 670 (190-3200 nm)

    (a)    Make: Jasco

    (b)   Model: UV-670

    (c)    Spectral Range: 190-3200 nm 

For Further Information Contact

Name : Central Analytical Laboratory
Incharge : Prof. Ramakrishnan Ganesan
Phone No.:- 040-66303602

An institution deemed to be a University estd. vide Sec.3 of the UGC Act,1956 under notification # F.12-23/63.U-2 of Jun 18,1964

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