Publications

Books Authored

Utpal Roy & Vishal Saxena, “A Handbook of Genetic Engineering”, 2007, Kalayni Publishers.
Laboratory Manual for Course BIO G642-Experimental Techniques.

Chapters Authored

Vishal Saxena, Shilpi Garg, 2011. “Plastids In Plasmodium – Significance In Malaria Treatment”, Current Topics in Biotechnology and Microbiology, LAP Lambert Academic Publishing AG & Co., Germany. pg 217 – 236.

Papers in Peer-Reviewed Journals

Alison Roth, Swamy R. Adapa, Min Zhang, Xiangyun Liao, Vishal Saxena, Raaven Goffe, Suzanne Li, Ratawan Ubalee, Gagandeep Singh Saagu, Zarna Rajeshkumar Pala, Shilpi Garg, Silas Davidson, Rays H.Y. Jiang, and John H. Adams (2018). Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host. Nature Scientific Reports, (In Press).
Gagandeep Singh Saggu, Shilpi Garg, Zarna Pala, Sanjay Kochar, Vishal Saxena (2018). Deciphering the role of IspD (2-C-methyl-D-erythritol 4-phosphate cytidyltransferase) enzyme as a potential therapeutic drug target against Plasmodium vivax. Gene, 675:240–253.
Zarna Pala, Vishal Saxena, Gagandeep Singh Saggu, Shilpi Garg (2018). Recent advances in the [Fe-S] Cluster Biogenesis (SUF) pathway functional in the apicoplast of Plasmodium. Trends in Parasitology, doi:10.1016/j.pt.2018.05.010
Gagandeep S. Saggu, Shilpi Garg, Zarna R. Pala, Sushil K. Yadav, Sanjay K. Kochar, Dhanpat K. Kochar, Vishal Saxena (2017) Characterization of 4-hydroxy-3-methylbut-2-en-1-yl diphosphatesynthase (IspG) from Plasmodium vivax and it’s potential as an antimalarial drug target. International Journal of Biological Macromolecules 96: 466–473. (Corresponding Author)
Gagandeep S. Saggu, Zarna R. Pala, Shilpi Garg, Vishal Saxena (2016). New Insight into Isoprenoids Biosynthesis Process and Future Prospects for Drug Designing in Plasmodium. Front. Microbiol. 7:1421. doi:10.3389/fmicb.2016.01421. (Corresponding Author)
Zarna Rajeshkumar Pala, Vishal Saxena, Gagandeep Singh Saggu, Sushil Kumar Yada, R.P. Pareek, Sanjay K. Kochar, Dhanpat K. Kochar, Shilpi Garg. (2016). Structural and functional characterization of an iron–sulfur cluster assembly scaffold protein-SufA from Plasmodium vivax, Gene, 585:159-165 (Corresponding Author in sharing).
Amit K Subudhi, Boopathi P Arunachalam, Shilpi Garg, Sheetal Middha, Jyoti Acharya, Deepak Pakalapati, Vishal Saxena, Aiyaz Mohammed, Harsha B Orekondy, Raja C Mugasimangalam, Paramendra Sirohi, Sanjay K Kochar, Dhanpat K Kochar, Ashis Das. (2014). An in vivo transcriptome data set of natural antisense transcripts from Plasmodium falciparum clinical isolates, Genomics Data, 2; 393-395.
P.A. Boopathi, Amit Kumar Subudhi, Shilpi Garg, Sheetal Middha, Jyoti Acharya, Deepak Pakalapati, Vishal Saxena, Mohammed Aiyaz, Bipin Chand, Raja C. Mugasimangalam, Sanjay K. Kochar, Parmendra Sirohi, Dhanpat K. Kochar, Ashis Das. (2014) Dataset of natural antisense transcripts in P. vivax clinical isolates derived using custom designed strand-specific microarray, Genomics Data, 2: 199–201.
Amit K Subudhi, Boopathi P Arunachalam, Shilpi Garg, Sheetal Middha, Jyoti Acharya, Deepak Pakalapati, Vishal Saxena, Aiyaz Mohammed, Harsha B Orekondy, Raja C Mugasimangalam, Paramendra Sirohi, Sanjay K Kochar, Dhanpat K Kochar (2014). Natural antisense transcripts in Plasmodium falciparum isolates from patients with complicated malaria, Experimental Parasitology, 141: 39-54.
P.A. Boopathi, Amit Kumar Subudhi, Shilpi Garg, Sheetal Middha, Jyoti Acharya, Deepak Pakalapati, Vishal Saxena, Mohammed Aiyaz, Bipin Chand, Raja C. Mugasimangalam, Sanjay K. Kochar, Parmendra Sirohi, Dhanpat K. Kochar, Ashis Das. (2013). Revealing natural antisense transcripts from Plasmodium vivax isolates: Evidence of genome regulation in complicated malaria. Infection, Genetics and Evolution, 20: 428-443.
Deepak Pakalapati, Shilpi Garg, Sheetal Middha, Jyoti Acharya, Amit K. Subudhi, P. A. Boopathi, Sanjay K. Kochar, Vishal Saxena, Dhanpat K. Kochar, Ashis Das. (2013). Development and evaluation of 28S rRNA based nested PCR assay for the detection of Plasmodium falciparum and Plasmodium vivax. Annal of Tropical Medicine and Parasitology. 06
Deepak Pakalapati, Shilpi Garg, Sheetal Middha, Abhishek Kochar, Amit K. Subudhi, P. A. Boopathi, Sanjay K. Kochar, Vishal Saxena, Rajendra P. Pareek, Jyoti Acharya, Dhanpat K. Kochar, Ashis Das. (2013). Comparative evaluation of microscopy, OptiMAL ® and 18S rRNA gene based multiplex PCR for detection of Plasmodium falciparum and Plasmodium vivax from field isolates of Bikaner, India. Asian Pacific Journal of Tropical Medicine 6(5): 346 - 351.
Shilpi Garg, Vishal Saxena, Vanshika Lumb, Deepak Pakalapati, Amit K. Subudhi, P.A. Boopathi, Shibasish Chowdhury, Sanjay K. Kochar, Dhanpat K. Kochar, Y. D. Sharma, Ashis Das. (2012). Novel mutations in the antifolate drug resistance marker genes among parasite isolates from severe Plasmodium vivax malaria group of patients. Experimental Parasitology, 132(4):410-16.
Vishal Saxena, Shilpi Garg, Jyotsana Tripathi, Sonal Sharma, Deepak P., Amit Subudhi, P. A. Boopathi, G.S.Saggu, Sanjay K. Kochar, Dhanpat K. Kochar, Ashis Das. (2012). Plasmodium vivax Apicoplast genome: A comparative analysis of major genes from Indian field isolates. Acta Tropica, 122(1):138-49. (Impact Factor: 2.5)
Dhanpat Kumar Kochar, Gajanand Singh Tanwar, Poonam Chand Khatri, Sanjay Kumar Kochar, Ghanshyam Singh Sengar, Anjana Gupta, Abhishek Kochar, Sheetal Middha, Jyoti Acharya, Vishal Saxena, Deepak Pakalapati, Shilpi Garg, and Ashish Das (2010). Clinical Features of Children Hospitalized with Malaria—A Study from Bikaner, Northwest India. Am. J. Trop. Med. Hyg., 83(5), 981-989. (Impact Factor: 2.795)
Dhanpat Kochar, Abhishek Kochar, Sheetal Middha, Jyoti Acharya, Gajanand Tanwar, Deepak P., Shilpi Garg, Vishal Saxena, Amit Subudhi, P. A. Boopathi, Ashis Das, Sanjay Kochar 2010. Thrombocytopenia in Plasmodium falciparum, Plasmodium vivax and mixed infection malaria: A study from Bikaner (northwestern India). Platelets 21(8):623-7. (Impact Factor: 2.271)
Shilpi Garg, Vishal Saxena, Swarna Kanchan, Pooja Sharma, Siddharth Mahajan, Dhanpat Kochar, Ashis Das. (2009). Novel point mutations in sulfadoxine resistance genes of Plasmodium falciparum from India. Acta Tropica, 110: 75 – 79. (Impact Factor: 2.5)
Dhanpat K. Kochar, Ashis Das, Sanjay K. Kochar, Vishal Saxena, Parmendra Sirohi, Shilpi Garg, Abhishek Kochar, Mahesh P. Khatri, Vikas Gupta (2009) Severe Plasmodium vivax Malaria: A Report on Serial Cases from Bikaner in Northwestern India. Am J Trop Med Hyg 80: 194-198. (Impact Factor: 2.795)
Vishal Saxena, Shilpi Garg, Sarita Ranjan, Dhanpat Kochar, Akash Ranjan, Ashis Das. (2007) Analysis of elongation factor Tu (tuf A) of apicoplast from Indian Plasmodium vivax isolates. Infection, Genetics and Evolution, 7: 618–626. (Impact Factor: 3.05)
Kochar, D., Saxena, V., Singh, N., Kochar, S., Kumar, V., Das, A. (2005). Plasmodium vivax malaria. Emerging Infectious Diseases, 11 (1):132 – 134. (Impact Factor: 6.859)
Kumar V.S., Ranjan S., Saxena, V., Rajesh, V., Kochar D., Roy S. K., Ranjan, A., Das A (2005). Plasmodium falciparum: Genetic Diversity of C – terminal region of MSP – 1 in isolates from the Indian subcontinent. Experimental Parasitology, 110: 384-388. (Impact Factor: 1.841)

Conference Proceedings

Zarna R. Pala, Shilpi Garg, Gagandeep Singh Saggu, Vishal Saxena. 2016 “Iron-sulphur cluster assembly in Plasmodium vivax: An insight into the SUF machinery.” 27th Annual Molecular Parasitology Meeting, at Woods Hole, Massachusetts, U.S.A., from September 18th – 22nd (Corresponding Author).
Shilpi Garg, Zarna R. Pala, Gagandeep S. Saggu, Vishal Saxena. 2016 “Elucidation of Suf pathway in Plasmodium vivax” presented at International Conference on Tropical Malaria and Medicine (ICTMM) 2016 at Brisbane, Australia from September 18th – 22nd.
Zarna R. Pala, Vishal Saxena, Gagandeep S. Saggu, Sushil K. Yadav, R. P. Pareek, Sanjay K. Kochar, Dhanpat K. Kochar, Shilpi Garg. 2016 “Structural and Functional Characterization of an Iron-Sulfur Cluster Assembly Scaffold Protein-SufA from Plasmodium vivax.” BITS Conference on Gene and Genome Regulation, at BITS Pilani, India, from February 18th – 20th (Corresponding Author in sharing; Ms Zarna, the PhD student won Best Poster Award).
Ramya R., Zarna R. Pala, Gagandeep S. Saggu, Shilpi Garg, Vishal Saxena. 2016 “Characterization of a novel t-RNA modifying enzyme from Plasmodium vivax.” BITS Conference on Gene and Genome Regulation”, at BITS Pilani, India, from 18th – 20th February, 2016 (Corresponding Author).
Ranita De, Vishal Saxena, Shilpi Garg. 2016 “Apicoplast division in Plasmodium: In-silico identification of potential candidate genes.” BITS Conference on Gene and Genome Regulation”, at BITS Pilani, India, from February 18th – 20th (Corresponding Author in sharing).
Vishal Saxena, Shilpi Garg, Zarna R. Pala, Sanjay K. Kochar, Dhanpat K. Kochar. 2014. "Characterization of the isoprenoid biosynthesis pathway enzymes from Indian Plasmodium vivax field isoates" in International Conference MEEGID XII – 12th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases at Bangkok, Thailand from December 10 – 13.
Gagandeep S. Saggu, Shilpi Garg, Zarna Pala, Sanjay K. Kochar, Vishal Saxena. 2014. “Characterization of 4-Hydroxy-3-Methylbut-2En-1Yl Diphosphate Synthase (GcpE) enzyme from Plasmodium vivax” in 8th National Congress of Indian Academy of Tropical Parasitology (TROPACON 2014) at Regional Medical Research Centre (RMRC), Dibrugarh, from November 20 – 22.
Vishal Saxena, Shilpi Garg, Zarna R. Pala, Sanjay K. Kochar. 2014. "Isoprenoid biosynthesis in Plasmodium vivax : few major players" in 25th National Conference of Parasitology on "Global Challenges in the Management of Parasitic Diseases" organised jointly by CSIR-CDRI and Indian Society of Parasitologists at CDRI, Lucknow from October 16 – 18.
Zarna Pala, Shilpi Garg, Sanjay K. Kochar, Dhanpat K. Kochar, Vishal Saxena. 2014 "Characterization of a nuclear encoded hypothetical protein involved in Fe-S cluster biogenesis in Plasmodium vivax" in 25th National Conference of Parasitology on "Global Challenges in the Management of Parasitic Diseases" organised jointly by CSIR-CDRI and Indian Society of Parasitologists at CDRI, Lucknow from October 16 – 18.
Gagandeep S. Saggu, Shilpi Garg, Zarna Pala, Sanjay K. Kochar, Vishal Saxena. 2014. “Characterization of Isoprenoids biosynthesis pathway enzymes from Indian Plasmodium vivax field isolates”. in Recent Trends and Future Prospects of Microbiology and Biotechnology organised by Shri Jagdishprasad Jhabarmal Tiberwala University, Jhunjhunu, Rajasthan, from March 03 – 04.
Zarna R. Pala, Shilpi Garg, Gagandeep S. Saggu, Vishal Saxena. 2014. “Characterization of nuclear encoded hypothetical proteins involved in Plasmodium vivax apicoplast metabolic pathways” in Recent Trends and Future Prospects of Microbiology and Biotechnology organized by Shri Jagdishprasad Jhabarmal Tiberwala University, Jhunjhunu, Rajasthan, from March 03 – 04.
Vishal Saxena, Shilpi Garg, Zarna R. Pala, Sanjay K. Kochar. 2013. "Characterization of the nuclear encoded hypothetical proteins targeted to the apicoplast in Plasmodium vivax" in International Conference on Advances in Plasmodium vivax malaria research at CosmoCaixa, Barcelona, Spain from May 28 -29.
Gagandeep S. Saggu, Zarna Pala, Shilpi Garg, Sanjay K. Kochar, Vishal Saxena. 2013. “Characterization of IspD enzyme from Indian Plasmodium vivax field isolates” in 24th National Congress of Parasitology held at Regional Medical Research Centre for Tribal’s, Jabalpur from April 27 – 29.
Zarna Pala, Ritika Lakhotia, Shilpi Garg, Gagandeep S. Saggu, Vishal Saxena. 2013. “Characterization and analysis of the RimM protein encoding gene in Plasmodium vivax” in National Conference on Fight Against Malaria: Prospects and Perspectives held at Centre for Biotechnology & Centre for Medical Biotechnology, Maharishi Dayanand University, Rohtak, Haryana on March 9th.
Vishal Saxena, Shilpi Garg, Ritika Lakhotia, Zarna R. Pala, Gagandeep S. Saggu, Swarna Kanchan. 2013. "Characterization of the nuclear encoded hypothetical proteins targeted to the apicoplast in Plasmodium vivax" in International Conference on Biomolecular Forms and Functions held at Indian Institute of Science, Bangalore, India from January 8 - 11.
Ritika Lakhotia, Shilpi Garg, Zarna Pala, Gagandeep S. Saggu, Akanksha Gupta, Vishal Saxena. 2012. Characterization of the RimM Protein encoding gene in P.vivax in International Conference on Microbial, Plant and Research at Mody Institute of Technology and Science, Sikar, Rajasthan, from March 29 – 31.
Vishal Saxena, Shilpi Garg, Gagandeep S. Saggu, Dhanpat K. Kochar, Sanjay K. Kochar, Ashis Das. “Molecular and Phylogenetic Analysis of Plasmodium vivax apicoplast genome” in International conference 22nd Molecular Parasitology Meeting held at Marine Biological Laboratory, Woods Hole, MA, USA from September 11 – 15, 2011.
Gagandeep S. Saggu, Ritika Lakhotia, Akansha Gupta, Shilpi Garg, Vishal Saxena. 2011. “Analysis of apicoplast targeted hypothetical proteins in Plasmodium vivax” in Contemporary Trends in Biological and Pharmaceutical Research, CTBPR– 2011 held at Birla Institute of Technology and Science, Pilani, India from March 12-13, 2011.
Vishal Saxena, Shilpi Garg, Dhanpat Kochar, Ashis Das. “The Apicoplast Genome of Plasmodium vivax” in International conference on Human Genome Organization (HUGO) – 2008 at HICC, Hyderabad from September 27 – 31, 2008.
Vishal Saxena, Shilpi Garg, Dhanpat Kochar, Ashis Das. “Plasmodium vivax – Apicoplast Genome” in International Workshop on Molecular Epidemiology & Immunology Of Malaria And Other Vector Borne Diseases at RMRCT, Jabalpur, M. P., INDIA from October 16 – 19, 2007, Pg 53.
Vishal Saxena, Shilpi Garg, Dhanpat Kochar, Ashis Das. “Apicoplast Genome of Plasmodium vivax” in 33rd Indian Immunology Society Conference at AIIMS, New Delhi, INDIA from January 28 – 31, 2007, Pg 96.
Saxena V., Ranjan A., Das A., “Characterisation of Plastid DNA genes from Plasmodium vivax” in Indo – Australian Conference on Biotechnology in Infectious Diseases at Kasturba Medical College, MAHE, Manipal, Bangalore, INDIA, from March 1 – 3, 2005, pg 55.
Saxena, V., Garg, S., Ranjan, S., Escalante, A., Kochar, D.K., Ranjan, A., Das, A. (2005). “Characterization of Plastid DNA elongation factor Tuf A from Plasmodium vivax”. At International Conference on Malaria, organized by National Institute of Malaria (ICMR) and NVBDCP, Delhi and MMV, Geneva at New Delhi, Nov. 4-6, Pg.229.
Saxena V., Ranjan A., Das A., “Characterisation of Plastid DNA elongation factor TufA from Plasmodium vivax” in National Symposium on Molecular Basis of Diseases, at IIT Madras, Chennai, INDIA, from January 8 – 9, 2005. Abstract in Advanced BIOTECH, 3(7): 27.
Saxena V., Ranjan A., Das A., “Characterisation of Apicoplast genome in Plasmodium vivax” in International Symposium on Emerging Trends in Genomics and Proteomics, Education and Research at Birla Institute of Technology & Science, Pilani, INDIA, 2003.
Garg S., Saxena V., Kochar D. K., Ranjan A., Das A., “Severe Malaria caused by Plasmodium vivax with Type 1 CSP repeats”. International Conference on Malaria, National Institute of Malaria (MRC) and ICMR, New Delhi, Nov. 3-5, 2005.
Garg S., Saxena V., Kochar D. K., Ranjan A., Das A., “Characterization of Dihydrofolate reductase gene of P.vivax severe malaria cases.” Poster Presentation in International Conference on Malaria, National Institute of Malaria (MRC) and ICMR, New Delhi, Nov. 3-5, 2005.
Kochar D. K., Singh N. V., Saxena V., Kochar S. K., Saxena S., Das A., “ Severe Plasmodium vivax malaria” International Conference on Malaria, National Institute of Malaria (MRC) and ICMR, New Delhi, Nov. 3-5, 2005.
Kumar V. S., Saxena V., Garg S., Roy S., Kochar D. K., Albin A., Pareekh R. P., Das A., “Plasmodium falciparum merozoite surface protein 119 humoral responses to allele specific variants from India”, International Conference on Malaria, National Institute of Malaria (MRC) and ICMR, New Delhi, Nov. 3-5, 2005.
Kochar D. K., Kasvan K., Sirohi P., Kochar S. K., Deepak P., Saxena V., Das A., “ A comparative study of regression of jaundice in malaria and acute viral hepatitis”, International Conference on Malaria, National Institute of Malaria (MRC) and ICMR, New Delhi, Nov. 3-5, 2005.
Subudhi AK., Boopathi PA. , Garg S., Pakalpati D., Saxena V., Middha S., Acharya J., Kochar D., Kochar S, Das A. “In vivo Transcriptome Study of Parasite Genes involved in Erythrocyte Sequestration and Immune Evasion from Severe P. falciparum field isolates”. Poster presentation in EMBO global lecture course on Molecular and Evolutionary Genetics of Malaria at National Institute of Malaria Research, Dwarka, New Delhi, 21st November to 4th December, 2010.
Shilpi Garg, Vishal Saxena, Dhanpat K. Kochar, Sanjay K. Kochar, Ashis Das, (2010). Molecular surveillance of drug resistance marker genes in Plasmodium vivax isolates causing severe manifestations from India. Poster Presentation in EMBO global lecture course on Molecular and Evolutionary Genetics of Malaria at National Institute of Malaria Research, Dwarka, New Delhi, 21st November to 4th December, 2010.
Garg S., Saxena V., Kochar D.K., Das A., (2011). Novel Point mutations in the antifolate drug resistance genes among Plasmodium falciparum and Plasmodium vivax isolates from India. Presented in 22nd Annual Molecular Parasitology Meeting held at Marine Biological Laboratory, Woods hole, MA, USA from September 11th to 15th 2011.
P.A. Boopathi, Amit Kumar Subudhi, Shilpi Garg, Deepak Pakalpati, Vishal Saxena, Sheetal Middha, Jyoti Acharya, Sanjay Kochar, Dhanpat Kochar, Ashis Das. The Transcriptome study of surface expressed variant antigen genes vir in Plasmodium vivax clinical isolates exhibiting severe manifestations. Poster Presentation in XI Symposium on Vector Borne Diseases, RMRCT, Jabalpur, 15th-17th October, 2011.
Subudhi AK., Boopathi PA. , Garg S., Pakalpati D., Saxena V., Middha S., Acharya J., Kochar D., Kochar S, Das A. “Plasmodium facliparum exportome - An in vivo transcriptome analysis of genes encoding exported protein from severe P.falciparum clinical isolates” XI Symposium on Vector Borne Diseases, RMRCT, Jabalpur, 15th-17th October, 2011.

Abstracts in Proceedings

Vishal Saxena, Shilpi Garg, Dhanpat Kochar, Ashis Das. “The apicoplast genome of Plasmodium vivax” ASTMH 59th Annual Meeting, Washington DC, November 17-22, 2010, published in Am J Trop Med Hyg, pg. 220.
Vishal Saxena, Shilpi Garg, Dhanpat Kochar, Ashis Das. “Plasmodium vivax - apicoplast genome” in ASTMH 58th Annual Meeting in Washington DC from 17-22 November 2009.

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