BITS Pilani

 
  • Page last updated on Wednesday, February 27, 2013

Research

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"A Structure is Worth Ten Thousand Gels"

The VISTA Lab

The VIbrio and STructural Analysis Lab (VISTA):

The Vista lab, at present has two main areas of research. The first involves the lifestyle switching of Vibrio cholerae and induction of virulence. Present research in this area involves investigation into a GGEEF domain protein from V. cholerae and characterization of a primary sigma factor from the same source. While structural studies involving X-ray crystallography is a major tool for these studies, molecular biology of V. cholerae and other biophysical methods are also being dealt with.

The second area is concerned with the prediction and validation of oncogenicity of microRNAs. While experimental methods to determine the involvement of microRNAs in oncogenic pathways is a time-consuming and extensive process, this lab has struck upon an innovative tool to predict and validate oncomeric microRNAs.

Additionally, the lab also deals with independent crystallization experiments from various natural sources, like Naja kaouthia (Indian monocellate cobra) and Moringa oleifera. The study of extremophilic protein architecture and stress adaptations are also some of the nascent areas the lab is interested in.
 

Research Projects

ONGOING PROJECTS:

 1
 Structure elucidation of VC0395_300 protein from
 Vibrio cholerae (leading to an alternate method of
checking Cholera epidemics)
 BRNS, DAE Rs. 23 lakhs
 2
 Physicochemical characterization of the Primary
 Sigma Factor of Vibrio cholerae
 DBT Rs. 31.75 lakhs
 3
 Expression, Purification and Characterization of
 GGEEprotein of Vibrio cholerae
 RIG, BITS Rs. 2 lakhs
 4
 Studies on putative GGEEF/Sensory box domain of
 Vibrio cholerae
 DST Rs. 21.8 lakhs
 5
 Biochemical and genetic characterization of 
 antimicrobia(candidacidal) peptides produced
 by the Antarctic strain Enterococcus faecalis
 APR 210 (as co-I)
 UGC Rs. 8 lakhs

Lab members

PhD scholars:
 
Ram K (CSIR-SRF)
Divya Bandekar  
Om Prakash Chauhan
 
Project scholars:
Malvika Sudhakar
Chirayu Garg 
Amit Dhanda
 

Other Interests

 

  • MicroRNA prediction and validation.
  • Toxin structures from Naja kaouthia venom.
  • Phage host interactions and specificity.
  • Hyperthermophile proteins and their interactions.
  • Stress related adaptations and xenobiotics.

 

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